Hotspot

trnN-GUU-trnR-ACG

IRa · 137327-137968 · 642 bp

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Pi 0.00159

MarkerSeek score 61.70

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 642
variable_sites: 2
pi: 0.00159
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	770-783 bp	1.0	79.8
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	807-820 bp	1.0	79.2
trnN-GUU-trnR-ACG_p3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	811-824 bp	1.0	79.0
trnN-GUU-trnR-ACG_p4	CCGTTGTTCCTCAGTAGCTC	TTTTAGGGTACTCCGGGGAA	55.0	50.0	58.0	57.7	824-837 bp	1.0	78.7
trnN-GUU-trnR-ACG_p5	CCGTTGTTCCTCAGTAGCTC	TTTTTAGGGTACTCCGGGGA	55.0	50.0	58.0	57.7	825-838 bp	1.0	78.7