Hotspot

ccsA-ndhD

SSC · 122178-122464 · 287 bp

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Pi 0.021

MarkerSeek score 61.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 287
variable_sites: 12
pi: 0.021
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGTTGCTTCTATGGGCTTTCT	CAGTTGACAAGGTTGAGGCT	42.9	50.0	57.6	58.0	439-440 bp	1.0	70.6
ccsA-ndhD_p2	TGTTGCTTCTATGGGCTTTCT	TCAGTTGACAAGGTTGAGGC	42.9	50.0	57.6	58.0	440-441 bp	1.0	70.6
ccsA-ndhD_p3	TGTTGCTTCTATGGGCTTTCT	ATCCGGATTGCGTTTTCTCA	42.9	45.0	57.6	57.9	463-464 bp	1.0	69.9
ccsA-ndhD_p4	TGTTGCTTCTATGGGCTTTCT	TCCGGATTGCGTTTTCTCAT	42.9	45.0	57.6	57.9	462-463 bp	1.0	69.9
ccsA-ndhD_p5	TGTTGCTTCTATGGGCTTTC	CAGTTGACAAGGTTGAGGCT	45.0	50.0	56.0	58.0	439-440 bp	1.0	66.3