Hotspot

ndhC-trnV-UAC

LSC · 53194-54355 · 1162 bp

Back to Parinari

Pi 0.00189

MarkerSeek score 61.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1162
variable_sites: 4
pi: 0.00189
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ATGGGGGATAAAACTCCGGA	GCATGTTGGGTCTTTGAAGC	50.0	50.0	58.1	57.9	1344-1360 bp	1.0	80.2
ndhC-trnV-UAC_p2	CTGGACCTTTGGTAATGGGG	GCATGTTGGGTCTTTGAAGC	55.0	50.0	57.9	57.9	1358-1374 bp	1.0	79.8
ndhC-trnV-UAC_p3	TCCGGAAATTCGAAATGCCA	GCATGTTGGGTCTTTGAAGC	45.0	50.0	58.2	57.9	1330-1346 bp	1.0	79.6
ndhC-trnV-UAC_p4	ATGGGGGATAAAACTCCGGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	1280-1296 bp	1.0	79.3
ndhC-trnV-UAC_p5	CTGGACCTTTGGTAATGGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.9	57.8	1294-1310 bp	1.0	78.9