Hotspot

ycf3-trnS-GGA

LSC · 47218-47694 · 477 bp

Back to Parinari

Pi 0.0053

MarkerSeek score 64.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 477
variable_sites: 5
pi: 0.0053
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	AGTTATCCGGGATCTAGGCA	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.3	57.8	511-536 bp	1.0	77.6
ycf3-trnS-GGA_p2	AGTTATCCGGGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.3	58.2	564-589 bp	1.0	77.5
ycf3-trnS-GGA_p3	CAGTTATCCGGGATCTAGGC	TTCCAATGCTACGCCTTGAA	55.0	45.0	57.0	57.8	512-537 bp	1.0	74.9
ycf3-trnS-GGA_p4	CAGTTATCCGGGATCTAGGC	ACGGAAAGAGAGGGATTCGA	55.0	50.0	57.0	58.2	565-590 bp	1.0	74.8
ycf3-trnS-GGA_p5	AGTTATCCGGGATCTAGGCA	AGTTCCAATGCTACGCCTTG	50.0	50.0	57.3	58.5	513-538 bp	1.0	74.8