Hotspot

psbM-trnD-GUC

LSC · 31194-32192 · 999 bp

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Pi 0.0015

MarkerSeek score 60.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 999
variable_sites: 3
pi: 0.0015
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACAAGAATGAACAGCGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.0	58.5	1115-1122 bp	1.0	76.7
psbM-trnD-GUC_p2	ATGAACAGCGCAGTAGCAAT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.3	58.5	1106-1113 bp	1.0	74.8
psbM-trnD-GUC_p3	TGAACAGCGCAGTAGCAATA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	1105-1112 bp	1.0	73.9
psbM-trnD-GUC_p4	ACAAGAATGAACAGCGCAGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.4	58.5	1112-1119 bp	1.0	73.7
psbM-trnD-GUC_p5	AATGAACAGCGCAGTAGCAA	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.5	58.5	1107-1114 bp	1.0	73.1