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petN-psbM

LSC · 30001-31088 · 1088 bp

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Pi 0.00184

MarkerSeek score 62.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1088
variable_sites: 4
pi: 0.00184
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	GCGCTGTTCATTCTTGTTCC	50.0	50.0	58.2	58.0	1224-1252 bp	1.0	80.0
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	GCGCTGTTCATTCTTGTTCC	50.0	50.0	57.6	58.0	1163-1191 bp	1.0	78.5
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	1233-1261 bp	1.0	78.2
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	1234-1262 bp	1.0	77.2
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	ACTGCGCTGTTCATTCTTGT	50.0	45.0	58.2	58.4	1227-1255 bp	1.0	77.1