Hotspot

trnH-GUG-psbA

LSC · 128-538 · 411 bp

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Pi 0.0328

MarkerSeek score 71.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 411
variable_sites: 20
pi: 0.0328
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCCTCTAGATCTAGCAGCTGT	50.0	52.4	57.9	58.1	465-486 bp	1.0	77.3
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	CCCTCTAGATCTAGCAGCTGT	50.0	52.4	58.2	58.1	466-487 bp	1.0	77.1
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	TCCCTCTAGATCTAGCAGCTG	50.0	52.4	57.9	57.8	466-487 bp	1.0	77.1
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	AGATCTAGCAGCTGTCGAAG	50.0	50.0	57.9	56.8	459-480 bp	1.0	77.1
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	TCCCTCTAGATCTAGCAGCTG	50.0	52.4	58.2	57.8	467-488 bp	1.0	76.9