Hotspot

trnL-UAG-ccsA

SSC · 113692-113742 · 51 bp

Back to Parietaria

Pi 0.068
MarkerSeek score 66.00
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
51
variable_sites
6
pi
0.068
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA TGCTGTAAAGTTGAAAATATCA 50.0 27.3 57.9 52.0 135-141 bp 1.0 45.2
trnL-UAG-ccsA_p2 GACACGCTGCTCTTAGGAAG TGCTGTAAAGTTGAAAATATCA 55.0 27.3 58.4 52.0 133-139 bp 1.0 45.2
trnL-UAG-ccsA_p3 TAGACACGCTGCTCTTAGGA TGCTGTAAAGTTGAAAATATCAT 50.0 26.1 57.9 52.7 135-141 bp 1.0 45.2
trnL-UAG-ccsA_p4 GGTAGACACGCTGCTCTTAG TGCTGTAAAGTTGAAAATATCA 55.0 27.3 57.8 52.0 137-143 bp 1.0 45.1
trnL-UAG-ccsA_p5 GTAGACACGCTGCTCTTAGG TGCTGTAAAGTTGAAAATATCA 55.0 27.3 57.8 52.0 136-142 bp 1.0 45.1