Hotspot

trnN-GUU-rpl32

SSC · 107939-112613 · 4675 bp

Back to Parietaria

Pi 0.0231
MarkerSeek score 49.80
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
SSC
length_bp
4675
variable_sites
197
pi
0.0231
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 TGCCGTTGGAAAAAGGAGAA TTTTTGTAGCGCTACATGCA 45.0 40.0 57.9 56.4 869-913 bp 1.0 73.8
trnN-GUU-rpl32_p2 TTGCCGTTGGAAAAAGGAGA TTTTTGTAGCGCTACATGCA 45.0 40.0 57.9 56.4 870-914 bp 1.0 73.8
trnN-GUU-rpl32_p3 GTTGCCGTTGGAAAAAGGAG TTTTTGTAGCGCTACATGCA 50.0 40.0 57.9 56.4 871-915 bp 1.0 73.2
trnN-GUU-rpl32_p4 AAAAAGGAGAAGCCCGACTC TTTTTGTAGCGCTACATGCA 50.0 40.0 57.8 56.4 860-904 bp 1.0 72.9
trnN-GUU-rpl32_p5 GAAAAAGGAGAAGCCCGACT TTTTTGTAGCGCTACATGCA 50.0 40.0 57.8 56.4 861-905 bp 1.0 72.9