Hotspot

rps12-clpP

LSC · 70159-70320 · 162 bp

Back to Parietaria

Pi 0.0412

MarkerSeek score 63.90

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 162
variable_sites: 12
pi: 0.0412
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	ATTGGCTGTCGTGTGTTTCT	CCCATTTCAGCGTCACAAAC	45.0	50.0	58.0	57.9	836-847 bp	1.0	85.9
rps12-clpP_p2	ATTGGCTGTCGTGTGTTTCT	CGGGCGAATTTATCCTGGAA	45.0	50.0	58.0	58.0	384-388 bp	0.6	67.0
rps12-clpP_p3	AGGAGTTAAGGGAAGGGCAT	GCTCACGGACTTGTTGATCT	50.0	50.0	58.0	57.9	620-625 bp	0.6	66.2
rps12-clpP_p4	AGGAGTTAAGGGAAGGGCAT	CGGGCGAATTTATCCTGGAA	50.0	50.0	58.0	58.0	766-771 bp	0.6	66.1
rps12-clpP_p5	AGGAGTTAAGGGAAGGGCAT	CCCATTTCAGCGTCACAAAC	50.0	50.0	58.0	57.9	1219-1224 bp	0.6	65.5