Hotspot

rps4-trnT-UGU

LSC · 46184-46699 · 516 bp

Back to Parietaria

Pi 0.0348

MarkerSeek score 67.80

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 516
variable_sites: 34
pi: 0.0348
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCCCAGACGACGTATTTTT	GATGGTCATCGGTTCGACTC	50.0	55.0	57.9	58.2	557-597 bp	1.0	86.7
rps4-trnT-UGU_p2	CCCCCAGACGACGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	588-628 bp	1.0	86.6
rps4-trnT-UGU_p3	CTGGGGCGTAATTGGTTTCT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.2	57.9	655-695 bp	1.0	85.7
rps4-trnT-UGU_p4	GTTTCCGGCTCTAGGCTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.2	57.9	629-669 bp	1.0	85.7
rps4-trnT-UGU_p5	CTGGGGCGTAATTGGTTTCT	GATGGTCATCGGTTCGACTC	50.0	55.0	58.2	58.2	624-664 bp	1.0	85.7