Hotspot

trnE-UUC-trnT-GGU

LSC · 30575-31222 · 648 bp

Back to Parietaria

Pi 0.0432

MarkerSeek score 70.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 648
variable_sites: 49
pi: 0.0432
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	745-789 bp	1.0	87.9
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	724-768 bp	1.0	87.6
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	721-765 bp	1.0	87.4
trnE-UUC-trnT-GGU_p4	CCAGCGTAGACATATTGCCA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	854-898 bp	1.0	87.0
trnE-UUC-trnT-GGU_p5	ACCAGCGTAGACATATTGCC	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	855-899 bp	1.0	86.9