Hotspot

petN-psbM

LSC · 28618-29306 · 689 bp

Back to Parietaria

Pi 0.0319

MarkerSeek score 63.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 689
variable_sites: 37
pi: 0.0319
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TCAGTCCCGATGGATCCAAA	50.0	50.0	58.2	58.4	1395-1402 bp	1.0	81.9
petN-psbM_p2	ATCCCCTTTTGACTATGCGC	TCAGTCCCGATGGATCCAAA	50.0	50.0	58.3	58.4	1492-1499 bp	1.0	80.2
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	CAAGGAGGGTGCTGGTTTAT	50.0	50.0	58.2	57.5	1197-1201 bp	0.6	62.0
petN-psbM_p4	TTCTGCTACTTCAAAGGCCG	TCAGTCCCGATGGATCCAAA	50.0	50.0	58.2	58.4	1623-1628 bp	0.6	61.8
petN-psbM_p5	TTCTGCTACTTCAAAGGCCG	CAAGGAGGGTGCTGGTTTAT	50.0	50.0	58.2	57.5	1423-1427 bp	0.6	61.2