Hotspot

psbI-trnS-GCU

LSC · 8048-8143 · 96 bp

Back to Parietaria

Pi 0.0583

MarkerSeek score 69.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 96
variable_sites: 11
pi: 0.0583
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GCGTAATCCTGGACGTGAAG	TTCGAATCCCTCTCTTTCCG	55.0	50.0	58.7	57.0	141-190 bp	1.0	72.3
psbI-trnS-GCU_p2	GCGTAATCCTGGACGTGAAG	GGGTTCGAATCCCTCTCTTT	55.0	50.0	58.7	56.9	144-193 bp	1.0	71.3
psbI-trnS-GCU_p3	GCGTAATCCTGGACGTGAAG	GGGTTCGAATCCCTCTCTTTC	55.0	52.4	58.7	58.1	144-193 bp	1.0	71.2
psbI-trnS-GCU_p4	GCGTAATCCTGGACGTGAAG	GGTTCGAATCCCTCTCTTTCC	55.0	52.4	58.7	58.1	143-192 bp	1.0	71.2
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGA	TTCGAATCCCTCTCTTTCCG	50.0	50.0	57.1	57.0	140-189 bp	1.0	70.7