Hotspot

trnK-UUU-rps16

LSC · 4251-4997 · 747 bp

Back to Parietaria

Pi 0.0404
MarkerSeek score 59.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
747
variable_sites
53
pi
0.0404
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGGTCTTCCAAACTCTACCG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1151-1184 bp 1.0 84.8
trnK-UUU-rps16_p2 CGGTCTTCCAAACTCTACCG TTTTCTTGAGCCGTACGAGG 55.0 50.0 58.0 57.9 1160-1193 bp 1.0 84.7
trnK-UUU-rps16_p3 AGACTCTCTGGCTTCCCTTT TTTTCTTGAGCCGTACGAGG 50.0 50.0 58.0 57.9 1728-1765 bp 1.0 84.2
trnK-UUU-rps16_p4 CGACATGCTGCTTTTTCCAG TTTTCTTGAGCCGTACGAGG 50.0 50.0 58.0 57.9 1202-1233 bp 0.6 65.2
trnK-UUU-rps16_p5 CGACATGCTGCTTTTTCCAG GCCGTACGAGGAGAAAACTT 50.0 50.0 58.0 57.9 1193-1224 bp 0.6 65.2