Hotspot

trnH-GUG-psbA

LSC · 75-244 · 170 bp

Back to Parietaria

Pi 0.078
MarkerSeek score 71.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
170
variable_sites
24
pi
0.078
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 ATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 52.6 55.0 56.9 57.9 232-274 bp 1.0 74.7
trnH-GUG-psbA_p2 ATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 52.6 55.0 56.9 57.9 235-277 bp 1.0 74.5
trnH-GUG-psbA_p3 ATCCACTTGGCTACATCCGC CCTCTAGACCTAGCTGCTGT 55.0 55.0 60.2 57.9 232-274 bp 1.0 74.0
trnH-GUG-psbA_p4 CCACTTGGCTACATCCGC CCTCTAGACCTAGCTGCTGT 61.1 55.0 58.2 57.9 230-272 bp 1.0 74.0
trnH-GUG-psbA_p5 ATCCACTTGGCTACATCCGC TTCCCTCTAGACCTAGCTGC 55.0 55.0 60.2 57.9 235-277 bp 1.0 73.8