Hotspot

rps2

LSC · 31109-31819 · 711 bp

Back to Pariana

Pi 0.00422

MarkerSeek score 61.20

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 3
pi: 0.00422
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TCAAGCTATTTCGGCTCTTTCT	GGCAGTTGTCCAAGAATTAGC	40.9	47.6	57.8	57.5	846-847 bp	1.0	50.8
rps2_p2	CCGTAATGGAATGGTAGGATGA	GGCAGTTGTCCAAGAATTAGC	45.5	47.6	57.4	57.5	801-802 bp	1.0	47.5
rps2_p3	TCCGTAATGGAATGGTAGGATG	GGCAGTTGTCCAAGAATTAGC	45.5	47.6	57.4	57.5	802-803 bp	1.0	47.5
rps2_p4	TCAAGCTATTTCGGCTCTTTCT	TCTAGGCAGTTGTCCAAGAA	40.9	45.0	57.8	56.1	850-851 bp	1.0	47.4
rps2_p5	TCAAGCTATTTCGGCTCTTTC	GGCAGTTGTCCAAGAATTAGC	42.9	47.6	56.3	57.5	846-847 bp	1.0	47.2