Hotspot

ndhG

SSC · 113126-113656 · 531 bp

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Pi 0

MarkerSeek score 28.10

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AAGAATTTTGACTCATTCTAAGT	AGTTTTAGTCAGTTCATGAAAAA	26.1	26.1	52.0	52.8	616 bp	1.0	40.0
ndhG_p2	TGAAATTCTAAGAATTTTGACTCA	AGTTTTAGTCAGTTCATGAAAAA	25.0	26.1	52.9	52.8	625 bp	1.0	40.0
ndhG_p3	TCTAAGAATTTTGACTCATTCTAAGT	AGTTTTAGTCAGTTCATGAAAAA	26.9	26.1	54.7	52.8	619 bp	1.0	40.0
ndhG_p4	AAGAATTTTGACTCATTCTAAGT	AGTTTTAGTCAGTTCATGAAAAAT	26.1	25.0	52.0	53.4	616 bp	1.0	40.0
ndhG_p5	TGAAATTCTAAGAATTTTGACTCA	AGTTTTAGTCAGTTCATGAAAAAT	25.0	25.0	52.9	53.4	625 bp	1.0	40.0