Hotspot

rbcL-psaI

LSC · 57290-58518 · 1229 bp

Back to Pariana

Pi 0.00814

MarkerSeek score 63.40

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1229
variable_sites: 10
pi: 0.00814
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CGAGCCGGTGGATAAACTAG	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.9	58.0	1327-1328 bp	1.0	79.3
rbcL-psaI_p2	CGAGCCGGTGGATAAACTAG	TTGCCGGAAATACTAAGCCC	55.0	50.0	57.9	58.0	1318-1319 bp	1.0	79.1
rbcL-psaI_p3	CGAGCCGGTGGATAAACTAG	AAGAAGCCATTGCAATTGCC	55.0	45.0	57.9	57.9	1333-1334 bp	1.0	78.7
rbcL-psaI_p4	TCGAGCCGGTGGATAAACTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	1328-1329 bp	1.0	78.5
rbcL-psaI_p5	TCGAGCCGGTGGATAAACTA	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.2	58.0	1319-1320 bp	1.0	78.4