Hotspot

rps4-trnT-UGU

LSC · 46853-47156 · 304 bp

Back to Pariana

Pi 0.0132

MarkerSeek score 51.30

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 304
variable_sites: 4
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CTCCCAGACGGCGTATTTTT	CGAGGGTCATCGGTTCAAAT	50.0	50.0	58.3	58.0	386 bp	1.0	78.5
rps4-trnT-UGU_p2	CTCCCAGACGGCGTATTTTT	TTGTAATGCGAGGGTCATCG	50.0	50.0	58.3	58.1	394 bp	1.0	78.2
rps4-trnT-UGU_p3	CTCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	416 bp	1.0	77.6
rps4-trnT-UGU_p4	GTGAGTCCTGGTAAAGCTCC	CGAGGGTCATCGGTTCAAAT	55.0	50.0	57.6	58.0	402 bp	1.0	77.5
rps4-trnT-UGU_p5	GTGAGTCCTGGTAAAGCTCC	TTGTAATGCGAGGGTCATCG	55.0	50.0	57.6	58.1	410 bp	1.0	77.2