Hotspot

atpI-atpH

LSC · 32803-33437 · 635 bp

Back to Pariana

Pi 0.00472

MarkerSeek score 61.50

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 635
variable_sites: 3
pi: 0.00472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	CGAATCCATGGAGGGTCATC	GCAATAACAGAAGCAGCAGC	55.0	50.0	57.8	58.1	695 bp	1.0	78.2
atpI-atpH_p2	CGAATCCATGGAGGGTCATC	GCAGCAATAACAGAAGCAGC	55.0	50.0	57.8	58.1	698 bp	1.0	78.2
atpI-atpH_p3	CGAATCCATGGAGGGTCATC	GCAGTACCTTGACCAACTCC	55.0	55.0	57.8	58.2	752 bp	1.0	77.4
atpI-atpH_p4	CGAATCCATGGAGGGTCATC	AAGCAGCAGCAATTAGTGGA	55.0	45.0	57.8	57.8	685 bp	1.0	77.3
atpI-atpH_p5	CGAATCCATGGAGGGTCATC	GCCAATCCAGCAGCAATAAC	55.0	50.0	57.8	57.8	707 bp	1.0	77.2