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petN-trnC-GCA

LSC · 18643-19574 · 932 bp

Back to Pariana

Pi 0.00429

MarkerSeek score 61.20

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 932
variable_sites: 4
pi: 0.00429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	AACGCGGATGAATACTTGCT	50.0	45.0	58.2	58.0	1078-1092 bp	1.0	78.9
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	CGCGGATGAATACTTGCTCT	50.0	50.0	58.2	58.1	1076-1090 bp	1.0	77.9
petN-trnC-GCA_p3	CCACTCCTCCCCCATACTAC	AACGCGGATGAATACTTGCT	60.0	45.0	58.3	58.0	1134-1148 bp	1.0	77.8
petN-trnC-GCA_p4	TCCACTCCTCCCCCATACTA	AACGCGGATGAATACTTGCT	55.0	45.0	58.4	58.0	1135-1149 bp	1.0	77.1
petN-trnC-GCA_p5	CCACTCCTCCCCCATACTAC	CGCGGATGAATACTTGCTCT	60.0	50.0	58.3	58.1	1132-1146 bp	1.0	76.9