Hotspot

psbM-petN

LSC · 17787-18552 · 766 bp

Back to Pariana

Pi 0.00539

MarkerSeek score 60.70

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 4
pi: 0.00539
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	42.9	50.0	57.6	58.2	905-928 bp	1.0	70.0
psbM-petN_p2	TGCTACTGCACTGTTCATTCT	GTAGTATGGGGGAGGAGTGG	42.9	60.0	57.6	58.3	849-872 bp	1.0	69.1
psbM-petN_p3	AAAACAGTCAGCCAAAACGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.4	58.2	850-873 bp	1.0	68.6
psbM-petN_p4	TGCTACTGCACTGTTCATTCT	TAGTATGGGGGAGGAGTGGA	42.9	55.0	57.6	58.4	848-871 bp	1.0	68.4
psbM-petN_p5	AAAACAGTCAGCCAAAACGA	GTAGTATGGGGGAGGAGTGG	40.0	60.0	56.4	58.3	794-817 bp	1.0	67.7