Hotspot

trnG-UCC-trnT-GGU

LSC · 13925-15407 · 1483 bp

Back to Pariana

Pi 0.00473

MarkerSeek score 61.10

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1483
variable_sites: 7
pi: 0.00473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	AGCATGGCATTACTTCGCTA	38.1	45.0	55.5	57.4	1622-1721 bp	1.0	47.2
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	TAGCATGGCATTACTTCGCT	38.1	45.0	55.5	57.4	1623-1722 bp	1.0	47.2
trnG-UCC-trnT-GGU_p3	ACGAATCACACTTTTACCACT	GCATGGCATTACTTCGCTAC	38.1	50.0	55.5	57.3	1621-1720 bp	1.0	46.8
trnG-UCC-trnT-GGU_p4	GAACGAATCACACTTTTACCACT	AGCATGGCATTACTTCGCTA	39.1	45.0	57.3	57.4	1624-1723 bp	1.0	46.1
trnG-UCC-trnT-GGU_p5	GAACGAATCACACTTTTACCACT	TAGCATGGCATTACTTCGCT	39.1	45.0	57.3	57.4	1625-1724 bp	1.0	46.1