Hotspot

psbK-psbI

LSC · 7338-7725 · 388 bp

Back to Pariana

Pi 0.0258

MarkerSeek score 69.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 388
variable_sites: 10
pi: 0.0258
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGTGGATGTTATGCCTGTCA	TTTTTACTCCTCACGCCCAG	50.0	50.0	58.0	57.8	581 bp	1.0	79.8
psbK-psbI_p2	CTTTTGTTTGGCAAGCTGCT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.1	57.8	537 bp	1.0	79.6
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TTTTTACTCCTCACGCCCAG	50.0	50.0	57.9	57.8	532 bp	1.0	79.6
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	529 bp	1.0	77.6
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	530 bp	1.0	77.6