Hotspot

psbD

LSC · 33980-35041 · 1062 bp

Back to Parathesis

Pi 0.000942

MarkerSeek score 59.30

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	CCATTACACTAAACGGGGCA	ACCAAGCAAAACCGGTAGTT	50.0	45.0	57.9	57.9	1235 bp	1.0	78.6
psbD_p2	GGCAGTACGCGAGAAATCAT	ACCAAGCAAAACCGGTAGTT	50.0	45.0	58.4	57.9	1219 bp	1.0	76.1
psbD_p3	CCATTACACTAAACGGGGCA	CGGTAGTTTCTTGGTCACGA	50.0	50.0	57.9	57.6	1223 bp	1.0	75.7
psbD_p4	CCATTACACTAAACGGGGCA	ACCGGTAGTTTCTTGGTCAC	50.0	50.0	57.9	57.5	1225 bp	1.0	74.8
psbD_p5	CCATTACACTAAACGGGGCA	CCGGTAGTTTCTTGGTCACG	50.0	55.0	57.9	58.6	1224 bp	1.0	74.5