Hotspot

rps15-ycf1

SSC · 125733-126139 · 407 bp

Back to Parathesis

Pi 0.00247

MarkerSeek score 48.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 407
variable_sites: 1
pi: 0.00247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AGACCCTGGGTTTTCCTCTT	ATGGCAGTCGTTTCAGTCTC	50.0	50.0	58.2	57.9	527-529 bp	1.0	78.4
rps15-ycf1_p2	AGACCCTGGGTTTTCCTCTT	GGCAGTCGTTTCAGTCTCTT	50.0	50.0	58.2	57.8	525-527 bp	1.0	77.9
rps15-ycf1_p3	TCAACAGACCCTGGGTTTTC	ATGGCAGTCGTTTCAGTCTC	50.0	50.0	57.6	57.9	532-534 bp	1.0	76.9
rps15-ycf1_p4	TCAACAGACCCTGGGTTTTC	GGCAGTCGTTTCAGTCTCTT	50.0	50.0	57.6	57.8	530-532 bp	1.0	76.3
rps15-ycf1_p5	AGACCCTGGGTTTTCCTCTT	AATGGCAGTCGTTTCAGTCT	50.0	45.0	58.2	57.4	528-530 bp	1.0	74.7