Hotspot

trnF-GAA-ndhJ

LSC · 49465-50197 · 733 bp

Back to Parathesis

Pi 0.00136

MarkerSeek score 59.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 1
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGATAATCATCCGCGCTTGA	55.0	45.0	58.0	57.5	916 bp	1.0	75.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGATAATCATCCGCGCTTGA	50.0	45.0	58.1	57.5	880 bp	1.0	75.5
trnF-GAA-ndhJ_p3	TCAAATCTGGTTCCTGGCAC	TGATAATCATCCGCGCTTGA	50.0	45.0	58.1	57.5	865 bp	1.0	75.2
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	TGATAATCATCCGCGCTTGA	55.0	45.0	57.9	57.5	904 bp	1.0	74.8
trnF-GAA-ndhJ_p5	AATGAGGATGATGCATCGGG	TGATAATCATCCGCGCTTGA	50.0	45.0	57.8	57.5	943 bp	1.0	74.3