Hotspot

atpH-atpI

LSC · 14640-15249 · 610 bp

Back to Parathesis

Pi 0.00328

MarkerSeek score 60.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 610
variable_sites: 2
pi: 0.00328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TTTTGCAACTTTAGCCGCTG	45.0	45.0	58.1	57.9	695 bp	1.0	78.3
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCCGCTG	50.0	45.0	57.9	57.9	753 bp	1.0	78.3
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	TTTTGCAACTTTAGCCGCTG	50.0	45.0	57.8	57.9	705 bp	1.0	77.8
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	TTTTGCAACTTTAGCCGCTG	55.0	45.0	58.2	57.9	757 bp	1.0	77.6
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCT	TTTTGCAACTTTAGCCGCTG	50.0	45.0	57.6	57.9	735 bp	1.0	75.7