Hotspot

trnI-CAU-rpl23

IRa · 149795-150139 · 345 bp

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Pi 0.0421

MarkerSeek score 65.30

Primer available yes

Label trnI-CAU-rpl23

Feature Metadata

label_name: trnI-CAU-rpl23
feature_type: igs
region: IRa
length_bp: 345
variable_sites: 8
pi: 0.0421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.55
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-rpl23_p1	GAATGGTTAAAGCGCCCAAC	TGATTTTTCGCCAATAACCGA	50.0	38.1	58.0	56.5	314-469 bp	1.0	65.8
trnI-CAU-rpl23_p2	CCTGTTAAGCATCCATGGCT	TGATTTTTCGCCAATAACCGA	50.0	38.1	57.9	56.5	334-489 bp	1.0	65.3
trnI-CAU-rpl23_p3	GAATGGTTAAAGCGCCCAAC	CCTAGTTGATCCTGATTCGACA	50.0	45.5	58.0	57.3	348-503 bp	1.0	64.2
trnI-CAU-rpl23_p4	GAATGGTTAAAGCGCCCAAC	TGTCCTAGTTGATCCTGATTCG	50.0	45.5	58.0	57.3	351-506 bp	1.0	64.2
trnI-CAU-rpl23_p5	GAATGGTTAAAGCGCCCAAC	CGACCCAATGCTTTATTTCTGT	50.0	40.9	58.0	57.3	370-525 bp	1.0	63.7