Hotspot

ycf2-trnI-CAU

IRa · 149639-149720 · 82 bp

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Pi 0.179

MarkerSeek score 70.80

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 82
variable_sites: 10
pi: 0.179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	TCCAGGAAACAGCTCTCAGA	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	578 bp	0.5	59.8
ycf2-trnI-CAU_p2	TCCAGGAAACAGCTCTCAGA	GTCCCATAATGCGTCCCATT	50.0	50.0	58.0	58.0	1167 bp	0.5	59.7
ycf2-trnI-CAU_p3	CTTCCGGATCCAGGAAACAG	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.0	58.0	586 bp	0.5	59.7
ycf2-trnI-CAU_p4	CTTCCGGATCCAGGAAACAG	GTCCCATAATGCGTCCCATT	55.0	50.0	58.0	58.0	1175 bp	0.5	59.6
ycf2-trnI-CAU_p5	TCCAGGAAACAGCTCTCAGA	GCCAATTCCAATAGACGGGT	50.0	50.0	58.0	57.9	651 bp	0.5	59.5