Hotspot

trnN-GUU-trnR-ACG

IRa · 128454-129101 · 648 bp

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Pi 0.0149

MarkerSeek score 71.60

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 648
variable_sites: 9
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	788-830 bp	1.0	82.4
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	698-740 bp	1.0	82.0
trnN-GUU-trnR-ACG_p3	GGGTTGAGAACGGGAATTGA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	787-829 bp	1.0	81.6
trnN-GUU-trnR-ACG_p4	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	702-744 bp	1.0	81.1
trnN-GUU-trnR-ACG_p5	TGGGTTGAGAACGGGAATTG	GGGACTCCTAGGAGCAGATC	50.0	60.0	58.1	58.4	836-878 bp	1.0	81.0