Hotspot

ycf1-trnN-GUU

IRa · 127870-128381 · 512 bp

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Pi 0.0631
MarkerSeek score 75.30
Primer available yes
Label ycf1-trnN-GUU

Feature Metadata

label_name
ycf1-trnN-GUU
feature_type
igs
region
IRa
length_bp
512
variable_sites
28
pi
0.0631
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnN-GUU_p1 ATTACCTGGTATAAACGGCA AGGATTCGAACCTACGACCA 40.0 50.0 54.1 58.2 528-595 bp 1.0 55.7
ycf1-trnN-GUU_p2 TTACCTGGTATAAACGGCAT AGGATTCGAACCTACGACCA 40.0 50.0 54.1 58.2 527-594 bp 1.0 55.7
ycf1-trnN-GUU_p3 ATTACCTGGTATAAACGGCA CCCCAAGTAGGATTCGAACC 40.0 55.0 54.1 57.7 536-603 bp 1.0 54.3
ycf1-trnN-GUU_p4 TTACCTGGTATAAACGGCAT CCCCAAGTAGGATTCGAACC 40.0 55.0 54.1 57.7 535-602 bp 1.0 54.3
ycf1-trnN-GUU_p5 ATTACCTGGTATAAACGGCAT AGGATTCGAACCTACGACCA 38.1 50.0 54.8 58.2 528-595 bp 1.0 52.8