Hotspot

trnL-UAG-ccsA

SSC · 112732-112820 · 89 bp

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Pi 0.163

MarkerSeek score 82.60

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 89
variable_sites: 14
pi: 0.163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TGGAATTGGTAGACACGCTG	ACGACTGAAACGACCGAAAA	50.0	45.0	57.9	58.1	221-227 bp	1.0	84.7
trnL-UAG-ccsA_p2	GTGGAATTGGTAGACACGCT	ACGACTGAAACGACCGAAAA	50.0	45.0	57.9	58.1	222-228 bp	1.0	84.7
trnL-UAG-ccsA_p3	TAGACACGCTGCTCTTAGGA	ACGACTGAAACGACCGAAAA	50.0	45.0	57.9	58.1	212-218 bp	1.0	84.7
trnL-UAG-ccsA_p4	GGTAGACACGCTGCTCTTAG	ACGACTGAAACGACCGAAAA	55.0	45.0	57.8	58.1	214-220 bp	1.0	84.1
trnL-UAG-ccsA_p5	GTAGACACGCTGCTCTTAGG	ACGACTGAAACGACCGAAAA	55.0	45.0	57.8	58.1	213-219 bp	1.0	84.1