Hotspot

trnI-CAU-ycf2

IRb · 86375-86456 · 82 bp

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Pi 0.179

MarkerSeek score 70.80

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 82
variable_sites: 10
pi: 0.179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	TCCAGGAAACAGCTCTCAGA	50.0	50.0	58.0	58.0	578 bp	0.5	59.8
trnI-CAU-ycf2_p2	GTCCCATAATGCGTCCCATT	TCCAGGAAACAGCTCTCAGA	50.0	50.0	58.0	58.0	1167 bp	0.5	59.7
trnI-CAU-ycf2_p3	GTTGGGCGCTTTAACCATTC	CTTCCGGATCCAGGAAACAG	50.0	55.0	58.0	58.0	586 bp	0.5	59.7
trnI-CAU-ycf2_p4	GTCCCATAATGCGTCCCATT	CTTCCGGATCCAGGAAACAG	50.0	55.0	58.0	58.0	1175 bp	0.5	59.6
trnI-CAU-ycf2_p5	GCCAATTCCAATAGACGGGT	TCCAGGAAACAGCTCTCAGA	50.0	50.0	57.9	58.0	651 bp	0.5	59.5