Hotspot

psbN-psbH

LSC · 75356-75453 · 98 bp

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Pi 0.0408

MarkerSeek score 72.40

Primer available yes

Label psbN-psbH

Feature Metadata

label_name: psbN-psbH
feature_type: igs
region: LSC
length_bp: 98
variable_sites: 4
pi: 0.0408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbH_p1	GGCGTACCCGGTAAAACTTA	TCAACGCTTTGTGTAGCCAT	50.0	45.0	57.6	58.1	184 bp	1.0	77.8
psbN-psbH_p2	TAAGGCGTACCCGGTAAAAC	TCAACGCTTTGTGTAGCCAT	50.0	45.0	57.6	58.1	187 bp	1.0	77.8
psbN-psbH_p3	GGCGTACCCGGTAAAACTTA	CAGTGGTTTCAAAAAGGCCC	50.0	50.0	57.6	57.8	242 bp	1.0	76.4
psbN-psbH_p4	TAAGGCGTACCCGGTAAAAC	CAGTGGTTTCAAAAAGGCCC	50.0	50.0	57.6	57.8	245 bp	1.0	76.4
psbN-psbH_p5	GGCGTACCCGGTAAAACTTA	CCTACAGCAGTTCGTCTTGG	50.0	55.0	57.6	58.3	223 bp	1.0	76.1