Hotspot

rps12-clpP

LSC · 71362-71533 · 172 bp

Back to Parashorea

Pi 0.0872

MarkerSeek score 73.50

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 15
pi: 0.0872
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	GATTGGCTGTCTTGCGTTTC	TGGAATTATTGATCTTATAGCAGA	50.0	29.2	58.0	53.4	245-258 bp	1.0	44.8
rps12-clpP_p2	AGGACATCCCCTAAGAGCAG	TGGAATTATTGATCTTATAGCAGA	55.0	29.2	58.2	53.4	284-297 bp	1.0	44.1
rps12-clpP_p3	GAGGACATCCCCTAAGAGCA	TGGAATTATTGATCTTATAGCAGA	55.0	29.2	58.2	53.4	285-298 bp	1.0	44.1
rps12-clpP_p4	CTCGTACATGTTCCTCGACG	TGGAATTATTGATCTTATAGCAGA	55.0	29.2	58.2	53.4	307-320 bp	1.0	43.8
rps12-clpP_p5	CCTCGTACATGTTCCTCGAC	TGGAATTATTGATCTTATAGCAGA	55.0	29.2	57.8	53.4	308-321 bp	1.0	43.8