Hotspot

rpl33-rps18

LSC · 68979-69546 · 568 bp

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Pi 0.0374

MarkerSeek score 69.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 568
variable_sites: 19
pi: 0.0374
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGGTTCAGGTTCCAAGACAA	GACCAGTCCACGTTTGAGTT	50.0	50.0	58.0	58.1	1481-1506 bp	1.0	84.8
rpl33-rps18_p2	CGGTTCAGGTTCCAAGACAA	ACCAGTCCACGTTTGAGTTC	50.0	50.0	58.0	58.1	1480-1505 bp	1.0	84.7
rpl33-rps18_p3	GGGGTTCATGGCCAAAAGTA	CTTTCTCAGCAGGAATCCCC	50.0	55.0	58.1	58.0	1353-1372 bp	1.0	84.6
rpl33-rps18_p4	CGGTTCAGGTTCCAAGACAA	CTTTCTCAGCAGGAATCCCC	50.0	55.0	58.0	58.0	1668-1695 bp	1.0	84.2
rpl33-rps18_p5	CGGTTCAGGTTCCAAGACAA	CAGTAATTCGCGACATGGGA	50.0	50.0	58.0	58.1	1814-1841 bp	1.0	83.9