Hotspot

ycf4-cemA

LSC · 61490-62281 · 792 bp

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Pi 0.0871

MarkerSeek score 81.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 66
pi: 0.0871
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GTCGCATCCTCCTACGATTC	CGTATCGATACAACCCGCAT	55.0	50.0	57.9	57.9	1325-1371 bp	1.0	85.3
ycf4-cemA_p2	GTCGCATCCTCCTACGATTC	CGGAAGGGTGGAAACAAGAA	55.0	50.0	57.9	58.0	1601-1647 bp	1.0	84.6
ycf4-cemA_p3	ATAGCGGGGTCTCGAAAAAC	TTGTGCGCCTTTAGCAATTG	50.0	45.0	58.0	57.9	1610-1656 bp	1.0	84.6
ycf4-cemA_p4	ATAGCGGGGTCTCGAAAAAC	CGTATCGATACAACCCGCAT	50.0	50.0	58.0	57.9	1629-1675 bp	1.0	84.5
ycf4-cemA_p5	ATAGCGGGGTCTCGAAAAAC	CGGAAGGGTGGAAACAAGAA	50.0	50.0	58.0	58.0	1905-1951 bp	1.0	84.1