Hotspot

psbZ-trnG-UCC

LSC · 36553-37222 · 670 bp

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Pi 0.0419

MarkerSeek score 79.20

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 670
variable_sites: 28
pi: 0.0419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTTCTCCTGATGGTTGGGTG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	57.9	57.8	824-884 bp	1.0	80.5
psbZ-trnG-UCC_p2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	827-887 bp	1.0	79.8
psbZ-trnG-UCC_p3	TTCTCCTGATGGTTGGGTGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	823-883 bp	1.0	79.4
psbZ-trnG-UCC_p4	CTTCTCCTGATGGTTGGGTG	TAAATACATTGCCTGGGCGG	55.0	50.0	57.9	58.3	863-923 bp	1.0	79.2
psbZ-trnG-UCC_p5	TCCTGATGGTTGGGTGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	820-880 bp	1.0	78.8