Hotspot

rpoC1-rpoB

LSC · 23434-23459 · 26 bp

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Pi 0.0385

MarkerSeek score 74.60

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 1
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	GGGTTTTGTCACCTCTCCAA	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.6	57.9	222 bp	1.0	76.7
rpoC1-rpoB_p2	GGGTTTTGTCACCTCTCCAA	TTCGATTGCTCGTTCGAGAA	50.0	45.0	57.6	57.7	244 bp	1.0	74.7
rpoC1-rpoB_p3	GGGTTTTGTCACCTCTCCAA	TTTCGATTGCTCGTTCGAGA	50.0	45.0	57.6	57.7	245 bp	1.0	74.7
rpoC1-rpoB_p4	GGGTTTTGTCACCTCTCCAA	CGATCTTTGGCTCTGGAACT	50.0	50.0	57.6	57.6	221 bp	1.0	74.3
rpoC1-rpoB_p5	GGGTTTTGTCACCTCTCCAA	TCGATTGCTCGTTCGAGAAT	50.0	45.0	57.6	57.4	243 bp	1.0	73.0