Hotspot

ndhA

SSC · 117987-120135 · 2149 bp

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Pi 0.00465

MarkerSeek score 50.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2149
variable_sites: 14
pi: 0.00465
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTGAGACAAGTCGTGAA	CCTCCGGGGTTTATTAATTTGC	40.0	45.5	55.2	57.7	2302-2305 bp	1.0	40.5
ndhA_p2	TTGTTTGAGACAAGTCGTGA	CCTCCGGGGTTTATTAATTTGC	40.0	45.5	55.2	57.7	2303-2306 bp	1.0	40.5
ndhA_p3	TTGTTTGAGACAAGTCGTGAA	CCTCCGGGGTTTATTAATTTGC	38.1	45.5	56.0	57.7	2303-2306 bp	1.0	40.5
ndhA_p4	TGTTTGAGACAAGTCGTGAAT	CCTCCGGGGTTTATTAATTTGC	38.1	45.5	55.8	57.7	2302-2305 bp	1.0	40.5
ndhA_p5	TGTTTGAGACAAGTCGTGAA	CTCCGGGGTTTATTAATTTGCA	40.0	40.9	55.2	57.0	2301-2304 bp	1.0	40.5