Hotspot

rpl32-trnL-UAG

SSC · 111644-112431 · 788 bp

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Pi 0.00593
MarkerSeek score 62.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
788
variable_sites
7
pi
0.00593
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCGTTAAAAGCTTTGT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 905-911 bp 1.0 79.6
rpl32-trnL-UAG_p2 GGCAGCGTTAAAAGCTTTGT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 906-912 bp 1.0 79.6
rpl32-trnL-UAG_p3 AAAGGATATTGGGCAGCGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 916-922 bp 1.0 79.5
rpl32-trnL-UAG_p4 AAAGGATATTGGGCAGCGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 917-923 bp 1.0 79.5
rpl32-trnL-UAG_p5 GGCAGCGTTAAAAGCTTTGT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 915-921 bp 1.0 78.6