Hotspot

ndhF-rpl32

SSC · 110430-111478 · 1049 bp

Back to Parasenecio

Pi 0.00254

MarkerSeek score 60.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1049
variable_sites: 4
pi: 0.00254
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	ACAAAGCTTTTAACGCTGCC	50.0	45.0	58.0	57.9	1243-1247 bp	1.0	79.0
ndhF-rpl32_p2	GTTGCCGTCGGAAAAAGAAG	AACGCTGCCCAATATCCTTT	50.0	45.0	58.0	57.9	1232-1236 bp	1.0	78.9
ndhF-rpl32_p3	TGCCGTCGGAAAAAGAAGAA	ACAAAGCTTTTAACGCTGCC	45.0	45.0	58.0	57.9	1241-1245 bp	1.0	78.9
ndhF-rpl32_p4	TTGCCGTCGGAAAAAGAAGA	ACAAAGCTTTTAACGCTGCC	45.0	45.0	58.0	57.9	1242-1246 bp	1.0	78.9
ndhF-rpl32_p5	TACTTTTTGTTGCCGTCGGA	ACAAAGCTTTTAACGCTGCC	45.0	45.0	58.0	57.9	1251-1255 bp	1.0	78.9