Hotspot

ndhC-trnV-UAC

LSC · 50294-51046 · 753 bp

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Pi 0.00974
MarkerSeek score 70.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
753
variable_sites
10
pi
0.00974
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCCTTTGCTAATCGGAGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.1 57.8 848-908 bp 1.0 76.0
ndhC-trnV-UAC_p2 TCGGAGCTAAAAATCCCGAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 56.9 57.8 837-897 bp 1.0 74.8
ndhC-trnV-UAC_p3 CCCTTTGCTAATCGGAGCTAA AGAAGGTCTACGGTTCGAGT 47.6 50.0 57.8 57.8 848-908 bp 1.0 73.8
ndhC-trnV-UAC_p4 CCCTTTGCTAATCGGAGCTA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.1 57.5 847-907 bp 1.0 73.3
ndhC-trnV-UAC_p5 GGCCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 57.9 50.0 58.3 57.8 850-910 bp 1.0 73.0