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atpI-atpH

LSC · 25089-26182 · 1094 bp

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Pi 0.00061

MarkerSeek score 59.20

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1094
variable_sites: 1
pi: 0.00061
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TTTTTGCAACTTTAGCCGCG	GGCAGAAACCAGTGGATTCA	45.0	50.0	58.5	58.1	1170-1171 bp	1.0	75.3
atpI-atpH_p2	TTTTTGCAACTTTAGCCGCG	TACCTTGACCAACTCCAGGT	45.0	50.0	58.5	57.9	1237-1238 bp	1.0	75.1
atpI-atpH_p3	TTTTTGCAACTTTAGCCGCG	AGCCAATCCAGCAGCAATAA	45.0	45.0	58.5	57.9	1197-1198 bp	1.0	74.8
atpI-atpH_p4	TTTTTGCAACTTTAGCCGCG	AATAACGGAAGCGGCAGAAA	45.0	45.0	58.5	58.2	1182-1183 bp	1.0	74.5
atpI-atpH_p5	TTTTTGCAACTTTAGCCGCG	GCAGTACCTTGACCAACTCC	45.0	55.0	58.5	58.2	1241-1242 bp	1.0	74.5