Hotspot

rps16-trnQ-UUG

LSC · 6351-7319 · 969 bp

Back to Parasenecio

Pi 0.00275

MarkerSeek score 61.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 969
variable_sites: 4
pi: 0.00275
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1034-1042 bp	1.0	79.2
rps16-trnQ-UUG_p2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1049-1057 bp	1.0	77.3
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1048-1056 bp	1.0	77.3
rps16-trnQ-UUG_p4	GCCAAGAGCACTTTCATTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.4	58.1	1097-1105 bp	1.0	75.1
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1038-1046 bp	1.0	74.5