Hotspot

trnK-UUU-rps16

LSC · 4438-5256 · 819 bp

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Pi 0.00488

MarkerSeek score 61.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 819
variable_sites: 6
pi: 0.00488
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	955 bp	1.0	76.6
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	954 bp	1.0	76.6
trnK-UUU-rps16_p3	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.8	58.3	1040 bp	1.0	76.1
trnK-UUU-rps16_p4	CAGTCGTGGTCTTCCAAACT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.8	58.3	1039 bp	1.0	76.0
trnK-UUU-rps16_p5	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	970 bp	1.0	75.9