primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4438	5256	955-955	1.000000	76.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4438	5256	954-954	1.000000	76.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.547945	4438	5256	1040-1040	1.000000	76.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CAGTCGTGGTCTTCCAAACT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.554139	4438	5256	1039-1039	1.000000	76.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.3	58.3	0.0	7.6	0.579483	4438	5256	970-970	1.000000	75.9
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6351	7319	1034-1042	1.000000	79.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6351	7319	1049-1057	1.000000	77.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6351	7319	1048-1056	1.000000	77.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GCCAAGAGCACTTTCATTCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.4	58.1	0.0	16.9	0.720034	6351	7319	1097-1105	1.000000	75.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6351	7319	1038-1046	1.000000	74.5
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCTGGATTACG	20	20	50.0	50.0	57.9	57.1	1.4	0.0	0.989589	7928	8378	587-588	1.000000	72.6
psbK-psbI_p2	psbK-psbI	2	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCTGGATTACG	20	20	45.0	50.0	58.3	57.1	1.4	0.0	1.242299	7928	8378	584-585	1.000000	70.6
psbK-psbI_p3	psbK-psbI	3	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCTGGATTACG	20	20	45.0	50.0	58.3	57.1	1.4	0.0	1.242299	7928	8378	585-586	1.000000	70.6
psbK-psbI_p4	psbK-psbI	4	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCTGGATTACG	20	20	45.0	50.0	58.3	57.1	1.4	0.0	1.242299	7928	8378	586-587	1.000000	70.6
psbK-psbI_p5	psbK-psbI	5	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGTCCTGGATTACG	20	21	50.0	47.6	57.9	57.8	1.4	0.0	1.282440	7928	8378	588-589	1.000000	70.3
trnC-GCA-petN_p1	trnC-GCA-petN	1	GTTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.192931	9523	10316	907-910	1.000000	80.1
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9523	10316	850-853	1.000000	79.9
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9523	10316	868-871	1.000000	77.7
trnC-GCA-petN_p4	trnC-GCA-petN	4	TGTTGTGTATCGTTTTGGCG	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.3	58.2	0.0	0.0	0.823536	9523	10316	908-911	1.000000	75.1
trnC-GCA-petN_p5	trnC-GCA-petN	5	GTTGTGTATCGTTTTGGCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.0	57.1	0.0	0.0	0.934367	9523	10316	906-909	1.000000	74.2
atpI-atpH_p1	atpI-atpH	1	TTTTTGCAACTTTAGCCGCG	GGCAGAAACCAGTGGATTCA	20	20	45.0	50.0	58.5	58.1	0.0	28.1	0.608023	25089	26182	1170-1171	1.000000	75.3
atpI-atpH_p2	atpI-atpH	2	TTTTTGCAACTTTAGCCGCG	TACCTTGACCAACTCCAGGT	20	20	45.0	50.0	58.5	57.9	0.0	0.0	0.630002	25089	26182	1237-1238	1.000000	75.1
atpI-atpH_p3	atpI-atpH	3	TTTTTGCAACTTTAGCCGCG	AGCCAATCCAGCAGCAATAA	20	20	45.0	45.0	58.5	57.9	0.0	0.0	0.666168	25089	26182	1197-1198	1.000000	74.8
atpI-atpH_p4	atpI-atpH	4	TTTTTGCAACTTTAGCCGCG	AATAACGGAAGCGGCAGAAA	20	20	45.0	45.0	58.5	58.2	0.0	1.4	0.701648	25089	26182	1182-1183	1.000000	74.5
atpI-atpH_p5	atpI-atpH	5	TTTTTGCAACTTTAGCCGCG	GCAGTACCTTGACCAACTCC	20	20	45.0	55.0	58.5	58.2	0.0	0.0	0.708039	25089	26182	1241-1242	1.000000	74.5
psaA-ycf3_p1	psaA-ycf3	1	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	20	20	55.0	50.0	57.8	58.3	18.5	0.0	0.487535	41331	42058	920-922	1.000000	76.4
psaA-ycf3_p2	psaA-ycf3	2	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	20	20	55.0	50.0	57.8	58.3	18.5	0.0	0.488764	41331	42058	922-924	1.000000	76.4
psaA-ycf3_p3	psaA-ycf3	3	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	20	20	55.0	45.0	57.8	57.5	18.5	0.0	0.716540	41331	42058	921-923	1.000000	74.6
psaA-ycf3_p4	psaA-ycf3	4	TTTTACTTCTGGTTCCGGCG	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.745852	41331	42058	855-857	1.000000	74.4
psaA-ycf3_p5	psaA-ycf3	5	TTTTACTTCTGGTTCCGGCG	CAAGCCGCTGAGTATTGGAA	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.747080	41331	42058	857-859	1.000000	74.4
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.273485	47891	48618	831-853	1.000000	71.8
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.275704	47891	48618	830-852	1.000000	71.8
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	20	21	50.0	47.6	58.1	57.7	0.0	0.0	1.349030	47891	48618	838-860	1.000000	71.2
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	20	21	50.0	47.6	58.1	57.5	0.0	3.3	1.537359	47891	48618	832-854	1.000000	69.7
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	20	21	50.0	47.6	58.1	58.6	0.0	3.3	1.647337	47891	48618	831-853	1.000000	68.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCCTTTGCTAATCGGAGCTA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.1	57.8	0.0	0.0	1.107971	50294	51046	848-908	1.000000	76.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCGGAGCTAAAAATCCCGAA	AGAAGGTCTACGGTTCGAGT	20	20	45.0	50.0	56.9	57.8	7.9	0.0	1.260777	50294	51046	837-897	1.000000	74.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCCTTTGCTAATCGGAGCTAA	AGAAGGTCTACGGTTCGAGT	21	20	47.6	50.0	57.8	57.8	0.0	0.0	1.391288	50294	51046	848-908	1.000000	73.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCCTTTGCTAATCGGAGCTA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.1	57.5	0.0	0.0	1.451743	50294	51046	847-907	1.000000	73.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGCCCTTTGCTAATCGGAG	AGAAGGTCTACGGTTCGAGT	19	20	57.9	50.0	58.3	57.8	0.0	0.0	1.489381	50294	51046	850-910	1.000000	73.0
rbcL_p1	rbcL	1	ACGCCTAATTCATGTCGAGT	TGGGCCGAGTTTCATTGAAA	20	20	45.0	45.0	57.0	58.0	0.0	7.7	0.973233	54765	56243	1569-1569	1.000000	72.6
rbcL_p2	rbcL	2	ACGCCTAATTCATGTCGAGT	TTGGGCCGAGTTTCATTGAA	20	20	45.0	45.0	57.0	58.0	0.0	0.0	0.973233	54765	56243	1570-1570	1.000000	72.6
rbcL_p3	rbcL	3	ACGCCTAATTCATGTCGAGT	ATTGGGCCGAGTTTCATTGA	20	20	45.0	45.0	57.0	57.8	0.0	0.0	1.175902	54765	56243	1571-1571	1.000000	70.9
rbcL_p4	rbcL	4	ACGCCTAATTCATGTCGAGT	GTTGGATTGGATTCGGCTCA	20	20	45.0	50.0	57.0	58.2	0.0	0.0	1.211333	54765	56243	1607-1607	1.000000	70.7
rbcL_p5	rbcL	5	ACGCCTAATTCATGTCGAGT	TGTTGGATTGGATTCGGCTC	20	20	45.0	50.0	57.0	58.2	0.0	0.0	1.211333	54765	56243	1608-1608	1.000000	70.7
accD-psaI_p1	accD-psaI	1	AAAAAGCGTTCTGAGTGAGT	AAAGAAGCCATTGCGATTGC	20	20	40.0	45.0	55.5	58.0	0.0	1.6	2.466839	58269	58934	804-804	1.000000	61.3
accD-psaI_p2	accD-psaI	2	AAAAGCGTTCTGAGTGAGTT	AAAGAAGCCATTGCGATTGC	20	20	40.0	45.0	55.5	58.0	0.0	1.6	2.466839	58269	58934	803-803	1.000000	61.3
accD-psaI_p3	accD-psaI	3	TCAACTCCACACTTTCTTTCCT	AAAGAAGCCATTGCGATTGC	22	20	40.9	45.0	57.5	58.0	0.0	1.6	2.504143	58269	58934	780-780	1.000000	61.0
accD-psaI_p4	accD-psaI	4	AAAAAGCGTTCTGAGTGAGTT	AAAGAAGCCATTGCGATTGC	21	20	38.1	45.0	56.3	58.0	0.0	1.6	2.681113	58269	58934	804-804	1.000000	59.5
accD-psaI_p5	accD-psaI	5	AAAAGCGTTCTGAGTGAGTT	TTGCGATTGCCGGAAATACT	20	20	40.0	45.0	55.5	58.3	0.0	8.0	2.721178	58269	58934	793-793	1.000000	59.2
psaI-ycf4_p1	psaI-ycf4	1	GCAATCGCAATGGCTTCTTT	TACTTGTTTTTCGAGCCCCC	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.100184	59046	59435	505-507	1.000000	80.0
psaI-ycf4_p2	psaI-ycf4	2	GCAATCGCAATGGCTTCTTT	GGATAACAGCCCAGCAGAAA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.129200	59046	59435	526-528	1.000000	79.7
psaI-ycf4_p3	psaI-ycf4	3	GCAATCGCAATGGCTTCTTT	AAAGGATAACAGCCCAGCAG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.135218	59046	59435	529-531	1.000000	79.7
psaI-ycf4_p4	psaI-ycf4	4	TAGGCCTAGTATTTCCGGCA	TACTTGTTTTTCGAGCCCCC	20	20	50.0	50.0	57.9	58.1	8.9	0.0	0.190853	59046	59435	522-524	1.000000	79.2
psaI-ycf4_p5	psaI-ycf4	5	TAGGCCTAGTATTTCCGGCA	GGATAACAGCCCAGCAGAAA	20	20	50.0	50.0	57.9	57.9	8.9	0.0	0.219869	59046	59435	543-545	1.000000	79.0
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	62268	63030	897-901	1.000000	77.2
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.9	57.6	0.0	4.1	0.416851	62268	63030	887-891	1.000000	77.2
petA-psbJ_p3	petA-psbJ	3	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.619192	62268	63030	935-939	1.000000	75.6
petA-psbJ_p4	petA-psbJ	4	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.620943	62268	63030	934-938	1.000000	75.5
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.624069	62268	63030	925-929	1.000000	75.5
rpl16-rps3_p1	rpl16-rps3	1	CTAGATGTGATCCAAGCGGG	GAGGGTAGGGTTCCACTACA	20	20	55.0	55.0	57.8	57.8	0.0	6.0	0.403704	80712	81947	1439-1439	1.000000	77.0
rpl16-rps3_p2	rpl16-rps3	2	GGCGACCTGCTTCTATTTGT	GAGGGTAGGGTTCCACTACA	20	20	50.0	55.0	58.3	57.8	0.0	6.0	0.503815	80712	81947	1459-1459	1.000000	76.2
rpl16-rps3_p3	rpl16-rps3	3	GTTCAAGTGCCTGAAGAGCA	GAGGGTAGGGTTCCACTACA	20	20	50.0	55.0	58.4	57.8	0.0	6.0	0.645140	80712	81947	1420-1420	1.000000	75.1
rpl16-rps3_p4	rpl16-rps3	4	CTAGATGTGATCCAAGCGGG	AGAGGGTAGGGTTCCACTAC	20	20	55.0	55.0	57.8	57.5	0.0	0.0	0.707475	80712	81947	1440-1440	1.000000	74.6
rpl16-rps3_p5	rpl16-rps3	5	GGGTTCAAGTGCCTGAAGAG	GAGGGTAGGGTTCCACTACA	20	20	55.0	55.0	58.5	57.8	0.0	6.0	0.714753	80712	81947	1422-1422	1.000000	74.5
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	ACAAAGCTTTTAACGCTGCC	20	20	50.0	45.0	58.0	57.9	17.5	9.5	0.166615	110430	111478	1243-1247	1.000000	79.0
ndhF-rpl32_p2	ndhF-rpl32	2	GTTGCCGTCGGAAAAAGAAG	AACGCTGCCCAATATCCTTT	20	20	50.0	45.0	58.0	57.9	17.5	0.0	0.176290	110430	111478	1232-1236	1.000000	78.9
ndhF-rpl32_p3	ndhF-rpl32	3	TGCCGTCGGAAAAAGAAGAA	ACAAAGCTTTTAACGCTGCC	20	20	45.0	45.0	58.0	57.9	17.5	9.5	0.177905	110430	111478	1241-1245	1.000000	78.9
ndhF-rpl32_p4	ndhF-rpl32	4	TTGCCGTCGGAAAAAGAAGA	ACAAAGCTTTTAACGCTGCC	20	20	45.0	45.0	58.0	57.9	17.5	9.5	0.177905	110430	111478	1242-1246	1.000000	78.9
ndhF-rpl32_p5	ndhF-rpl32	5	TACTTTTTGTTGCCGTCGGA	ACAAAGCTTTTAACGCTGCC	20	20	45.0	45.0	58.0	57.9	17.5	9.5	0.183373	110430	111478	1251-1255	1.000000	78.9
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGCAGCGTTAAAAGCTTTGT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.9	58.0	13.5	0.0	0.142032	111644	112431	905-911	1.000000	79.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGCAGCGTTAAAAGCTTTGT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.9	58.0	13.5	0.0	0.143402	111644	112431	906-912	1.000000	79.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAAGGATATTGGGCAGCGTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.151706	111644	112431	916-922	1.000000	79.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAAGGATATTGGGCAGCGTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.153076	111644	112431	917-923	1.000000	79.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGCAGCGTTAAAAGCTTTGT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.9	57.9	13.5	0.0	0.258478	111644	112431	915-921	1.000000	78.6
ndhA_p1	ndhA	1	TGTTTGAGACAAGTCGTGAA	CCTCCGGGGTTTATTAATTTGC	20	22	40.0	45.5	55.2	57.7	0.0	0.0	5.124973	117987	120135	2302-2305	1.000000	40.5
ndhA_p2	ndhA	2	TTGTTTGAGACAAGTCGTGA	CCTCCGGGGTTTATTAATTTGC	20	22	40.0	45.5	55.2	57.7	0.0	0.0	5.124973	117987	120135	2303-2306	1.000000	40.5
ndhA_p3	ndhA	3	TTGTTTGAGACAAGTCGTGAA	CCTCCGGGGTTTATTAATTTGC	21	22	38.1	45.5	56.0	57.7	0.0	0.0	5.325534	117987	120135	2303-2306	1.000000	40.5
ndhA_p4	ndhA	4	TGTTTGAGACAAGTCGTGAAT	CCTCCGGGGTTTATTAATTTGC	21	22	38.1	45.5	55.8	57.7	0.0	0.0	5.545524	117987	120135	2302-2305	1.000000	40.5
ndhA_p5	ndhA	5	TGTTTGAGACAAGTCGTGAA	CTCCGGGGTTTATTAATTTGCA	20	22	40.0	40.9	55.2	57.0	0.0	0.0	5.801797	117987	120135	2301-2304	1.000000	40.5
rps15-ycf1_p1	rps15-ycf1	1	TCTGGAGAATTAGGAAAGGGA	AGAATTGAGGCTTTAGAGACTGT	21	23	42.9	39.1	55.4	57.2	0.0	0.0	7.382490	125592	125711	305-305	1.000000	40.5
rps15-ycf1_p2	rps15-ycf1	2	CTGGAGAATTAGGAAAGGGAAGA	AGAATTGAGGCTTTAGAGACTGT	23	23	43.5	39.1	57.3	57.2	0.0	0.0	7.454123	125592	125711	304-304	1.000000	40.5
rps15-ycf1_p3	rps15-ycf1	3	TCTGGAGAATTAGGAAAGGGAAG	AGAATTGAGGCTTTAGAGACTGT	23	23	43.5	39.1	57.3	57.2	0.0	0.0	7.454123	125592	125711	305-305	1.000000	40.5
rps15-ycf1_p4	rps15-ycf1	4	TCTGGAGAATTAGGAAAGGGAA	AGAATTGAGGCTTTAGAGACTGT	22	23	40.9	39.1	56.2	57.2	0.0	0.0	7.593475	125592	125711	305-305	1.000000	40.5
rps15-ycf1_p5	rps15-ycf1	5	TGGAGAATTAGGAAAGGGAAGA	AGAATTGAGGCTTTAGAGACTGT	22	23	40.9	39.1	56.2	57.2	0.0	0.0	7.593475	125592	125711	303-303	1.000000	40.5