Hotspot

rpl2

LSC · 87779-89274 · 1496 bp

Back to Parasassafras

Pi 0

MarkerSeek score 25.30

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 1496
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GGAATGCCCACGAATCTGAT	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	2609 bp	1.0	79.7
rpl2_p2	ATAATGGTAGATGCCCGGGA	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	2249 bp	1.0	79.7
rpl2_p3	GGAATGCCCACGAATCTGAT	TGAATTTGGGTCACTTGCGA	50.0	45.0	58.0	58.0	2661 bp	1.0	79.6
rpl2_p4	ATAATGGTAGATGCCCGGGA	TGAATTTGGGTCACTTGCGA	50.0	45.0	58.0	58.0	2301 bp	1.0	79.5
rpl2_p5	GGAATGCCCACGAATCTGAT	ACGGGGACATTCAATAAGGC	50.0	50.0	58.0	57.9	2542 bp	1.0	79.4